ELISA test for measuring progesterone (P4) in bovine milk utilises unique high affinity anti-progesterone antibody which is highly specific.

This test can be used for:

• Routine fertility management
• Checking for pregnancy loss
• Research into bovine reproduction and its management

Features of Ridgeway Science’s Progesterone ELISA test:

• • Progesterone range of approx. 0-30ng/ml

• Low cross-reactivity with other steroids and hormones so no false results.
• High-throughput format with results in 1.5 hours or less.
• 1 hour incubation recommended.
• Chromogenic substrate is not light sensitive.
• 96 wells, allowing large numbers of samples to be tested.
• Freshly prepared milk standards are available with 3-month shelf life for direct sample comparison.
• Long-established assay, cited in many research papers.
• Top-of-the-range monoclonal antibody with a high affinity for progesterone.
• No dilution of samples; can apply straight onto assay.
• 10ul sample size
• Use for both natural and synthetic progesterone
• It can be read at 405nm, 550 or 570nm with differing substrates (available at request). The best results found at 550nm.

The bovine reproductive cycle lasts approximately 21 days with progesterone levels lowest during oestrus and then climbing gradually after oestrus to a plateau generally above 10 ng/mL progesterone in the non-pregnant animal.

In the pregnant cow the level of progesterone rises following AI and is maintained, normally over 10 ng/mL until parturition.

Progesterone has a short half-life and so levels drop rapidly for example at approximately 21 days post-AI if pregnancy is not established. This can be used as a non-pregnancy test.

Cost

Price – £350 per kit (includes one 96 well plate and reagents) inc VAT plus carriage 

References
1.Van der Lende T et al, 2020. Infrequent Milk Progesterone Measurements in Daughters Enable Bull Selection for Cow Fertility. J. Dairy Sci. 87:3953–3957