Progesterone ELISA Plates

Progesterone ELISA Plates

Background of our ELISA plates


Ridgeway Science has a 30-year pedigree in producing gold standard progesterone ELISAs, used heavily by vets and within research institutes. Our ELISAs provide an  fast and accurate measurement of an animal’s progesterone (P4) level in milk, serum or plasma.

Our customers find this especially useful in studying cattle: investigating how the oestrous cycle translates into timing of insemination and ovulation. The assay can be used to research P4 in other species and is a very handy tool for looking at reproductive disease.

Our ELISAs have also been used as a benchmark test to compare other new and upcoming on-farm aids for heat detection, such as our product P4Gold.

How does the progesterone assay work?


The ELISA is a competitive indirect solid phase assay. The 96-well microplate is coated with a monoclonal antibody boasting very high specificity for progesterone.

Small volumes of milk (e.g. dairy cows, goats, sheep) or serum/plasma (e.g. horses, dogs, camels) can be used as test samples. They are added in conjunction with an enzyme conjugate and left to incubate.

Excess is washed off and addition of a substrate produces a colour change inversely proportional to the quantity of progesterone in the sample tested. This means a strong colour equals very low progesterone and vice versa.

Our progesterone ELISAs are not species specific, so can be used with a wide range of animals.

Our Products

Ridgeway Science have been manufacturing Progesterone ELISA plates for over 30 years. Our current Milk ELISA has been on the market for 10+ years and has been used by countless research institutes, vets and other customers. This ELISA focuses on progesterone levels typically seen in cycling cows, with a 0-50ng/ml.

We currently only supply bovine milk standards (as an add on) but can provide advice on how to make standards for other species or provide buffer standards.

Features of the Milk ELISA

  • Progesterone range of approx. 0-50ng/ml
  • Low cross reactivity with other steroids and hormones so no false results.
  • High throughput format with results in 1.5hrs or less.
  • 1 hr incubation recommended.
  • Chromogenic substrate not light sensitive.
  • 96 wells allowing large volumes of sample testing.
  • Freshly prepared milk standards available with 3 month shelf life for direct sample comparison.
  • Long established assay, cited in many research papers.
  • Top of the range monoclonal antibody with high affinity for progesterone.
  • No dilution of samples, can apply straight onto assay.
  • 10ul sample size
  • Use for both natural and synthetic progesterone
  • Can be read at 405nm, 550 or 570nm with differing substrates (available at request). Best results found at 550nm.

Milk ELISA Price List 

Product Price
550/570nm Milk ELISA £100
405nm Milk ELISA £105
Bovine milk standards £40

Our Progesterone ELISA for use with plasma or serum makes use of the same chemistry as our Milk ELISA . This assay can be used with any species and its uses range from timing of breeding/insemination, pregnancy, diagnosis of reproductive disease.

Features of the Plasma/Serum ELISA

  • Progesterone range of approx. 0-20ng/ml
  • Low cross reactivity with other steroids and hormones so no false results.
  • High throughput format with results in 3.5hrs or less.
  • 3 hr incubation recommended.
  • Chromogenic substrate not light sensitive.
  • 96 wells allowing large volumes of sample testing.
  • Frozen plasma and serum standards available for some species for direct sample comparison.
  • Long established assay, cited in many research papers.
  • Top of the range monoclonal antibody with high affinity for progesterone.
  • No dilution of samples, can apply straight onto assay.
  • 10ul sample size
  • Can be used to test samples from cows, pigs, sheep, dogs, camels, horses, goats.
  • Can be read at 405nm, 550 or 570nm with differing substrates (available at request). Best results found at 550nm.

Plasma/Serum ELISA Price list

Product Price
550/570nm Plasma/Serum ELISA £100
405nm Plasma/Serum ELISA £105
Species specific plasma or serum standards £50
ridgeway science progesterone testing

To order any of our products please contact us on +44 1594 530809 or email us at enquiries@ridgewayscience.co.uk. We look forward to hearing from you!