Ridgeway science is pleased to offer an ELISA for detecting Equine Chorionic Gonadotropin (eCG), previously known as Pregnant Mare’s Serum Gonadotropin (PMSG). eCG is typically administered to livestock for hormonal synchronisation to aid in ovulation and synchronised timing of insemination for a flock.

The presence of eCG production by a mare indicates the activity of endometrial cups, which form during pregnancy. However these cups can remain active after a foetus is lost, so other methods must also be used to ensure no false positives.

This assay can be utilised for equine research of reproductive disorders, as a tool for pregnancy detection/reproductive monitoring utilised by veterinarians or breeders, and for testing and quality control measures in eCG production facilities.

How it works

The Ridgeway Science eCG ELISA is a 96-well solid phase sandwich assay using a polyclonal anti-eCG coating. Your sample containing eCG may first need to be diluted as the range for this assay is 0-1IU/mL, meaning the assay is highly sensitive. The diluted sample is added and incubated. After an incubation stage, the excess sample is washed off and an HRP conjugate is added. After another incubation stage, the unbound conjugate is washed off and the chromogenic substrate is added. The addition of stop solution gives a colour change proportional to the quantity of eCG in your diluted sample. Multiply by your dilution factor for the final results.